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Air sampling – Reading the results

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  • #71382
    Fiona de Sousa
    Participant

    Author:
    Fiona de Sousa

    Position:

    Organisation:

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    Dear members,

    I know that air sampling in a new building is a contentious issue but we are currently undergoing it as part of the commissioning process for new operating theatres and one of the difficulties I face is people asking for the acceptable limit of certain organisms. Aside from fungal organisms I have been unable to find any references to guide me on specific organisms counts.

    I would like to hear people’s views on the isolation of skin or environmental flora when doing this sampling – how many CFU would be acceptable per air sample ?

    Fiona De Sousa
    Infection Prevention & Control Coordinator
    Sydney Adventist Hospital
    Fiona.Desousa@sah.org.au
    185 Fox Valley Road, Wahroonga, NSW, 2076

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    #71384
    Matthias Maiwald (KKH)
    Participant

    Author:
    Matthias Maiwald (KKH)

    Position:

    Organisation:

    State:

    Dear Fiona,

    Here at our institution, we are currently using this reference:

    Dharan S, Pittet D. Environmental controls in operating theatres. J Hosp Infect. 2002 Jun;51(2):79-84.
    http://www.ncbi.nlm.nih.gov/pubmed/12090793

    Realising that it is very difficult to set and apply acceptable CFU limits, and there always will be an arbitrary component to this.

    Best regards, Matthias.


    Matthias Maiwald, MD, FRCPA
    Consultant in Microbiology
    Adj. Assoc. Prof., Natl. Univ. Singapore
    Department of Pathology and Laboratory Medicine
    KK Women’s and Children’s Hospital
    100 Bukit Timah Road
    Singapore 229899
    Tel. +65 6394 8725 (Office)
    Tel. +65 6394 1389 (Laboratory)
    Fax +65 6394 1387

    Dear members,

    I know that air sampling in a new building is a contentious issue but we are currently undergoing it as part of the commissioning process for new operating theatres and one of the difficulties I face is people asking for the acceptable limit of certain organisms. Aside from fungal organisms I have been unable to find any references to guide me on specific organisms counts.

    I would like to hear people’s views on the isolation of skin or environmental flora when doing this sampling – how many CFU would be acceptable per air sample ?

    Fiona De Sousa
    Infection Prevention & Control Coordinator
    Sydney Adventist Hospital
    Fiona.Desousa@sah.org.au
    185 Fox Valley Road, Wahroonga, NSW, 2076

    If you are not the intended recipient you are hereby notified that any dissemination, distribution or reproduction of this message
    is prohibited. If you have received this message in error please notify the sender immediately, then destroy the original message.
    Any views expressed in this message are solely those of the individual sender, except where the sender is specifically authorised
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    _____________________________________________________________________
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    #71388
    Lindy Ryan
    Participant

    Author:
    Lindy Ryan

    Position:

    Organisation:

    State:

    Dear Fiona

    Not sure what others have used but I have found this document I have attached of use and nicely set out and easy to follow and so used it as some of my reference review and basis for the commissioning of our new operating theatres here a couple of years ago that was accepted by our management and micro here as part of Quality control.

    I also still went along with the commissioning team & watched and monitored the pressure & flow readings of the central sterile store room, to OT room to anaesthetic bay to corridors as part of airflow monitoring baseline given they were brand new (they had a wiz bang snake looking device…better than the dry ice smoke test I had used previously ….and actually provide a reading…I did feel modern!!)

    I didn’t check baseline starting humidity & temperature of each room that was sampled…. but would do this now I think of it & given we have had complaints it would have been good to add them to my micro sampling results to provide a better overall understanding on the environment that was being sampled at the time as part of our Quality control for future reference not just cleanliness / appropriateness at opening- As4187 provides guides on acceptable limits for storage of sterile stock which is why I used it as my reference point. I also was present for the witness testing for the HEPA filter installation and commissioning testing. Finally I did a visual inspection / audit at the same time as the air sampling using the OT audit tool we had been using

    Again this is just what we did with the little expertise we had and information I could locate to help …more than happy if any has a more scientific or practical ideas to share – I may have done too much or missed something but I was none the wiser and my management seemed satisfied. So will be interesting to see if anyone else has anything to share that I can perhaps use for next time (we are always building something new here !!)

    Cheers

    Lindy

    Lindy Ryan

    Infection Prevention & Control Clinical Nurse Consultant | Nepean Hospital, NBM LHD
    Infection control Service, PO Box 63 Penrith, 2751, NSW
    Tel (02) 4734 2228 | Fax (02) 4734 2517 | lindy.ryan@health.nsw.gov.au
    http://www.health.nsw.gov.au

    “wise and humane management of the patient is the best safeguard against infection”
    Florence Nightingale circa 1860

    [http://internal.health.nsw.gov.au/communications/e-signatures/images/NSW-Health-Nepean-Blue-Mountains-LHD.jpg]

    Dear members,

    I know that air sampling in a new building is a contentious issue but we are currently undergoing it as part of the commissioning process for new operating theatres and one of the difficulties I face is people asking for the acceptable limit of certain organisms. Aside from fungal organisms I have been unable to find any references to guide me on specific organisms counts.

    I would like to hear people’s views on the isolation of skin or environmental flora when doing this sampling – how many CFU would be acceptable per air sample ?

    Fiona De Sousa
    Infection Prevention & Control Coordinator
    Sydney Adventist Hospital
    Fiona.Desousa@sah.org.au
    185 Fox Valley Road, Wahroonga, NSW, 2076

    If you are not the intended recipient you are hereby notified that any dissemination, distribution or reproduction of this message
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    #71392
    Michael Wishart
    Participant

    Author:
    Michael Wishart

    Position:

    Organisation:

    State:
    NSW

    Hi Fiona

    I have found it very hard to get a definite consensus on this within Australia. This document from Dept of Health in WA is a reasonable starting point for internal discussions in your facility to come to an agreement over what is ‘acceptable’.

    http://www.health.wa.gov.au/CircularsNew/circular.cfm?Circ_ID12021

    Cheers
    Michael

    Michael Wishart
    Infection Control Coordinator
    Holy Spirit Northside Private Hospital
    627 Rode Road, Chermside, Qld 4032
    t: (07) 3326 3068 | f: (07) 3607 2226
    e: Michael.Wishart@svha.org.au
    w:www.holyspiritnorthside.org.au
    Please consider the environment before printing this email

    [http://www.interactivejam.com.au/images/ACIPC-conference.jpg]

    Dear members,

    I know that air sampling in a new building is a contentious issue but we are currently undergoing it as part of the commissioning process for new operating theatres and one of the difficulties I face is people asking for the acceptable limit of certain organisms. Aside from fungal organisms I have been unable to find any references to guide me on specific organisms counts.

    I would like to hear people’s views on the isolation of skin or environmental flora when doing this sampling – how many CFU would be acceptable per air sample ?

    Fiona De Sousa
    Infection Prevention & Control Coordinator
    Sydney Adventist Hospital
    Fiona.Desousa@sah.org.au
    185 Fox Valley Road, Wahroonga, NSW, 2076

    If you are not the intended recipient you are hereby notified that any dissemination, distribution or reproduction of this message
    is prohibited. If you have received this message in error please notify the sender immediately, then destroy the original message.
    Any views expressed in this message are solely those of the individual sender, except where the sender is specifically authorised
    by Adventist HealthCare Limited to state that they are the views of Adventist HealthCare Limited.
    _____________________________________________________________________
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    ______________________________________________________________________
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    #71394
    Michael Wishart
    Participant

    Author:
    Michael Wishart

    Position:

    Organisation:

    State:
    NSW

    Oops – the WA document I cited is apparently under review, and shouldn’t be taken as current best practice.

    Apologies.

    There is obviously a need for more robust and evidenced guidance on this within Australia.

    Cheers
    Michael

    Michael Wishart
    Infection Control Coordinator
    Holy Spirit Northside Private Hospital
    627 Rode Road, Chermside, Qld 4032
    t: (07) 3326 3068 | f: (07) 3607 2226
    e: Michael.Wishart@svha.org.au
    w:www.holyspiritnorthside.org.au
    Please consider the environment before printing this email

    [http://www.interactivejam.com.au/images/ACIPC-conference.jpg]

    Hi Fiona

    I have found it very hard to get a definite consensus on this within Australia. This document from Dept of Health in WA is a reasonable starting point for internal discussions in your facility to come to an agreement over what is ‘acceptable’.

    http://www.health.wa.gov.au/CircularsNew/circular.cfm?Circ_ID12021

    Cheers
    Michael

    Michael Wishart
    Infection Control Coordinator
    Holy Spirit Northside Private Hospital
    627 Rode Road, Chermside, Qld 4032
    t: (07) 3326 3068 | f: (07) 3607 2226
    e: Michael.Wishart@svha.org.au
    w:www.holyspiritnorthside.org.au
    Please consider the environment before printing this email

    [http://www.interactivejam.com.au/images/ACIPC-conference.jpg]

    Dear members,

    I know that air sampling in a new building is a contentious issue but we are currently undergoing it as part of the commissioning process for new operating theatres and one of the difficulties I face is people asking for the acceptable limit of certain organisms. Aside from fungal organisms I have been unable to find any references to guide me on specific organisms counts.

    I would like to hear people’s views on the isolation of skin or environmental flora when doing this sampling – how many CFU would be acceptable per air sample ?

    Fiona De Sousa
    Infection Prevention & Control Coordinator
    Sydney Adventist Hospital
    Fiona.Desousa@sah.org.au
    185 Fox Valley Road, Wahroonga, NSW, 2076

    If you are not the intended recipient you are hereby notified that any dissemination, distribution or reproduction of this message
    is prohibited. If you have received this message in error please notify the sender immediately, then destroy the original message.
    Any views expressed in this message are solely those of the individual sender, except where the sender is specifically authorised
    by Adventist HealthCare Limited to state that they are the views of Adventist HealthCare Limited.
    _____________________________________________________________________
    This e-mail has been scanned for viruses by Symantec Hosted Services
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    ______________________________________________________________________
    For the purposes of protecting the integrity and security of the SVHA network and the information held on it, all emails to and from any email address on the “svha.org.au” domain (or any other domain of St Vincent’s Health Australia Limited or any of its related bodies corporate) (an “SVHA Email Address”) will pass through and be scanned by the Symantec.cloud anti virus and anti spam filter service. These services may be provided by Symantec from locations outside of Australia and, if so, this will involve any email you send to or receive from an SVHA Email Address being sent to and scanned in those locations.
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    For the purposes of protecting the integrity and security of the SVHA network and the information held on it, all emails to and from any email address on the “svha.org.au” domain (or any other domain of St Vincent’s Health Australia Limited or any of its related bodies corporate) (an “SVHA Email Address”) will pass through and be scanned by the Symantec.cloud anti virus and anti spam filter service. These services may be provided by Symantec from locations outside of Australia and, if so, this will involve any email you send to or receive from an SVHA Email Address being sent to and scanned in those locations.
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    #71402
    John Ferguson
    Participant

    Author:
    John Ferguson

    Position:

    Organisation:

    State:

    Dear Fiona

    We also base our approach on Dharan

    I did put together an approach on the WIKI – see http://hicsigwiki.asid.net.au/index.php?titleOperating_theatre_commissioning_-_Microbiological

    It would be useful to agree a national standard for this!

    Kind regards
    John

    Dr John Ferguson
    Director, Infection Prevention & Control, Hunter New England Health

    [cid:image001.jpg@01CFC5FB.5251CEF0]

    Dear Fiona,

    Here at our institution, we are currently using this reference:

    Dharan S, Pittet D. Environmental controls in operating theatres. J Hosp Infect. 2002 Jun;51(2):79-84.
    http://www.ncbi.nlm.nih.gov/pubmed/12090793

    Realising that it is very difficult to set and apply acceptable CFU limits, and there always will be an arbitrary component to this.

    Best regards, Matthias.


    Matthias Maiwald, MD, FRCPA
    Consultant in Microbiology
    Adj. Assoc. Prof., Natl. Univ. Singapore
    Department of Pathology and Laboratory Medicine
    KK Women’s and Children’s Hospital
    100 Bukit Timah Road
    Singapore 229899
    Tel. +65 6394 8725 (Office)
    Tel. +65 6394 1389 (Laboratory)
    Fax +65 6394 1387

    Dear members,

    I know that air sampling in a new building is a contentious issue but we are currently undergoing it as part of the commissioning process for new operating theatres and one of the difficulties I face is people asking for the acceptable limit of certain organisms. Aside from fungal organisms I have been unable to find any references to guide me on specific organisms counts.

    I would like to hear people’s views on the isolation of skin or environmental flora when doing this sampling – how many CFU would be acceptable per air sample ?

    Fiona De Sousa
    Infection Prevention & Control Coordinator
    Sydney Adventist Hospital
    Fiona.Desousa@sah.org.au
    185 Fox Valley Road, Wahroonga, NSW, 2076

    If you are not the intended recipient you are hereby notified that any dissemination, distribution or reproduction of this message
    is prohibited. If you have received this message in error please notify the sender immediately, then destroy the original message.
    Any views expressed in this message are solely those of the individual sender, except where the sender is specifically authorised
    by Adventist HealthCare Limited to state that they are the views of Adventist HealthCare Limited.
    _____________________________________________________________________
    This e-mail has been scanned for viruses by Symantec Hosted Services
    Scanning Services – powered by MessageLabs. For further information
    visit http://www.messagelabs.com
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    #71404
    Glenys Harrington
    Participant

    Author:
    Glenys Harrington

    Position:
    Consultant

    Organisation:
    Infection Control Consultancy (ICC)

    State:

    Hi Fiona,

    Can you elaborate further on when you will be doing the air sampling?

    I’m assuming:

    . after Engineering ventilation parameters met and

    . before any occupation of the facility (i.e. staff/stock &
    equipment) and

    . after the ventilation has been running in the unoccupied facility
    for a 24hr period?

    Regards

    Glenys

    Glenys Harrington

    Consultant

    Infection Control Consultancy (ICC)

    PO Box 5202

    Middle Park

    Victoria, 3206

    Australia

    H: +61 3 96902216

    M: +61 404 816 434

    infexion@ozemail.com.au

    ABN 47533508426

    Of Fiona de Sousa

    Dear members,

    I know that air sampling in a new building is a contentious issue but we
    are currently undergoing it as part of the commissioning process for new
    operating theatres and one of the difficulties I face is people asking for
    the acceptable limit of certain organisms. Aside from fungal organisms I
    have been unable to find any references to guide me on specific organisms
    counts.

    I would like to hear people’s views on the isolation of skin or
    environmental flora when doing this sampling – how many CFU would be
    acceptable per air sample ?

    Fiona De Sousa

    Infection Prevention & Control Coordinator

    Sydney Adventist Hospital

    Fiona.Desousa@sah.org.au

    185 Fox Valley Road, Wahroonga, NSW, 2076

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    #71406
    Fiona de Sousa
    Participant

    Author:
    Fiona de Sousa

    Position:

    Organisation:

    State:

    Hi Glenys,

    Your assumptions are correct.
    Kind regards,

    Fiona De Sousa
    Infection Prevention & Control Coordinator
    Sydney Adventist Hospital
    Fiona.Desousa@sah.org.au
    185 Fox Valley Road, Wahroonga, NSW, 2076

    Hi Fiona,

    Can you elaborate further on when you will be doing the air sampling?

    I’m assuming:

    * after Engineering ventilation parameters met and

    * before any occupation of the facility (i.e. staff/stock & equipment) and

    * after the ventilation has been running in the unoccupied facility for a 24hr period?

    Regards

    Glenys

    Glenys Harrington
    Consultant
    Infection Control Consultancy (ICC)

    PO Box 5202
    Middle Park
    Victoria, 3206
    Australia
    H: +61 3 96902216
    M: +61 404 816 434
    infexion@ozemail.com.au
    ABN 47533508426

    Dear members,

    I know that air sampling in a new building is a contentious issue but we are currently undergoing it as part of the commissioning process for new operating theatres and one of the difficulties I face is people asking for the acceptable limit of certain organisms. Aside from fungal organisms I have been unable to find any references to guide me on specific organisms counts.

    I would like to hear people’s views on the isolation of skin or environmental flora when doing this sampling – how many CFU would be acceptable per air sample ?

    Fiona De Sousa
    Infection Prevention & Control Coordinator
    Sydney Adventist Hospital
    Fiona.Desousa@sah.org.au
    185 Fox Valley Road, Wahroonga, NSW, 2076

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